Rotor-Gene Q® Real-Time PCR Cycler

QiagenIndustrie Qiagen

THe unique Centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currentle available. Each tube spin in chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the samples is illuminated and the flourescent signal is rapidly corrected from a single, short optical pathway this thermal and and optical uniformity result is sensitive, precise, and fast real-time PCR analysis. It also eliminates sample-to-sample variations and edge effecs. These ara unavoidable intraditional block-based instruments due to temperature gradients across the block multiple, complex optical pathway.

The rotary design delivers:

  • Well-to-well variation below ±0.01°C (20 times less than block cyclers)
  • Uniform detection eliminating the need for ROX reference dye
  • Fast ramping and negligible equilibration for short run times
  • Complete confidence in your result

Whether your assay is based on intercalating dyes such SYBR Green, probes such as hydrolysis (TaqMan) or hybridization (FRET) probes, or multiplex chemistries, the Rotor-Gene Q meets your requirements. With up to 6 channels spinning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table “Channels for optical detection”). In addition, the software allows you to create news  excitation/detection wavelenghts combination, which means that the Rotor-Gene Q is compatible with dyes you may may use in the future.

 

Expand your research with HRM. High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enomous scientific interest. HRM characterizes double-standard PCR product based on their dissociation (melting) bahvior. It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. PCR products can be discriminated according to sequence, length, GC content  or strand complementarrity, down to single base-pair changes. previously unknown and even complex  sequence variation can be readily detected and characterized in a robust and straightforward way. The rotary design of the Rotor-Gene Q and its outstanding thermal and optical performance are highly suited to HRM.

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